Pgps3 Unique Restriction Sites |work| -

Just remember: no SalI, no EcoRV, and always verify your map. When in doubt, a quick virtual digest takes 30 seconds and saves three days of failed ligations.

: When sequencing from the transposon ends to identify insertion sites, unique restriction sites nearby serve as reliable reference points for mapping the relative position of the "hop" into target DNA. pgps3 unique restriction sites

If you received PGPS3 from a collaborator or bought a modified version (e.g., PGPS3-EGFP), do this: Just remember: no SalI, no EcoRV, and always verify your map

) within the Transprimer, allowing for dual selection during the mutagenesis process. Vector Database - pGPS3 - Addgene If you received PGPS3 from a collaborator or

| Restriction Enzyme | Position (approx.) | Recognition Sequence | Best Use Case | |-------------------|-------------------|----------------------|----------------| | | 1,234 | G^AATTC | Insert cloning (5' end) | | BamHI | 1,567 | G^GATCC | Insert cloning (3' end) | | HindIII | 2,345 | A^AGCTT | Backbone linearization | | XbaI | 789 | T^CTAGA | Subcloning from other vectors | | SacI | 2,890 | GAGCT^C | Terminator swaps | | PstI | 3,101 | CTGCA^G | Rare cutter for large inserts | | KpnI | 456 | GGTAC^C | Directional cloning with SacI | | NotI | 2,567 | GC^GGCCGC | For GC-rich inserts (rare cutter) |