Fcap Array Software ((hot)) -

: Interpolates sample concentrations by comparing MFI values against a standard curve generated using mathematical models like 4P or 5P Logistic .

The software's primary role is to manage microbead-based multiplex assays. By utilizing the fluorescence intensities of different bead populations, it can simultaneously identify and quantify multiple analytes within a single sample—up to 72 different analytes per sample in some versions. fcap array software

: The software calculates the concentration of analytes by comparing sample data against a standard curve. It supports advanced mathematical models like 4-parameter (4P) and 5-parameter (5P) logistic regression for high-accuracy fitting. : Interpolates sample concentrations by comparing MFI values

Robust FCAP software incorporates real-time QC: : The software calculates the concentration of analytes

Quantification is impossible without a standard curve. The software ingests data from a series of standards with known analyte concentrations (typically 7-10 points, serially diluted). FCAP software applies either a or 5-Parameter Logistic (5-PL) regression model. These models are superior to linear regression because they account for the sigmoidal shape of immunoassay response—asymptotic at low and high concentrations. The software automatically flags outliers, calculates R² values, and provides confidence intervals. A well-constructed standard curve (R² > 0.99) is the hallmark of a valid run.