: Supports counting within a specific Region of Interest (ROI) or using a black-and-white mask to isolate target areas.
While ITCN is a classic tool, it is no longer actively maintained. For more complex images or those requiring deep learning-based segmentation, many researchers have transitioned to newer ImageJ/Fiji plugins like StarDist or Cellpose, which often handle overlapping cells more accurately than traditional peak-finding methods. ITCN: Image-based Tool for Counting Nuclei itcn imagej plugin
Combine ITCN with the Batch Processor script: : Supports counting within a specific Region of
: ITCN can automatically count nuclei within an image or a selected region of interest. This feature significantly speeds up the analysis process compared to manual counting. itcn imagej plugin